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Processed fruit juice ready to drink: screening acute toxicity at the cellular level Biological Sciences
Silva, Erick Leal da; Sales, Ila Monize Sousa; Santos, Fabelina Karollyne Silva dos; Peron, Ana Paula.
The present study evaluated the acute toxicity at the cellular level of processed juice ready for consumption Orange and Grape flavors, produced by five companies with significant influence on the food market of South American countries, especially in Brazil. This evaluation was performed in root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, directly with marketed liquid preparations. Based on the results, it was found that fruit juices, of all companies considered, promoted significant antiproliferative effect to root meristems at the exposure time of 24 hours and resulted in at both exposure times, statistically significant number of mitotic spindle changes and chromosomal breaks. Therefore, under the study conditions, all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Industrialized juice; Cytotoxicity; Genotoxicity; Mutagenicity; Meristem tissue.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33699
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Toxicity at the cellular level of artificial synthetic flavorings Biological Sciences
Sales, Ila Monize Sousa; Oliveira, Jussara Damascena de; Santos, Fabelina Karollyne Silva dos; Feitoza, Lidiane de Lima; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents Cookie and Tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hours using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of Cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of Tutti-Frutti caused no change in cell division...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30751
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Toxicity in food flavorings at the cellular level associated with each other at different doses Biological Sciences
Silva, Sara Iolanda Oliveira da; Santana, Gleuvânia Marques; Sales, Ila Monize Sousa; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aroma and flavor additives; Cytotoxicity; Genotoxicity A. cepa Citogenética.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475
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